| Summary: | 碩士 === 中山醫學大學 === 醫學研究所 === 98 === ABSTRACT
Colorectal cancer (CRC) is one of the most malignancies in Taiwan. Early symptom is not easy to diagnosis, but lately stage almost has metastasis and worse prognosis. CEA level is considered as a predictive factor in colorectal cancer patients. But elevated CEA was found in other malignancies and inflammation. So, it is very important to found up another biological tumor marker to instead of limited CEA.
In our previous study, Daxx affected Tcf4 activity and regulated cell proliferation and differentiation in colon cells. CD24 and EHF were candidate genes regulated by Daxx expressing which were identified by gene microarray. Because of higher CD24 expression is associated with metastasis and shorter survival in various malignant tumors. On the other hand, Individual EHF can inhibit carcinogenesis by transcriptional regulation. It is our interesting to understand correlation between Daxx, β-catenin and CD24 or EHF in CRC carcinogenesis.
First, in clinical 106 paired CRC tissues analysis, a negative correlation was observed between CEA and Daxx. There were 39 patients (45.3%) with elevated CEA, but 58 patients (67.4%) with decreased Daxx expression, so Daxx may have diagnostic potential instead to CEA. In protein analysis, positive correlation was observed between CD24 or EHF with Daxx by Pearson’s correlation coefficient test (p=0.041, p=0.017). Reduction of EHF expression was relative to depth of invasion (p<0.05).
In cell lines, immunofluorescence analysis was performed, and CD24 was translocated from membrane to cytosol in Daxx knockdown stabled Hct116. On the other hand, cellular growth and cellular migration were increased rather than control cells. With our investing- ation above, Daxx may play a role to influence a prognostic factor, CD24 or EHF in CRC model, and further evidences will be investigated .
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