| Summary: | 碩士 === 中山醫學大學 === 醫學檢驗暨生物技術學系碩士班 === 98 === There are four isozymes of alkaline phosphatase (ALP) in the human, tissue nonspecific alkaline phosphatase (TNAP), placental alkaline phosphatase (PLAP), germ cell alkaline phosphatase (GCAP), and intestinal alkaline phosphatase (IAP). The recent studies showed that TNAP was not only involved in the mineralization, but also involved in calcification. From our previous study, it is known that TNAP expressed at early developmental stages of zebrafish, especially at kidney, the adult organ for hematopoiesis that is similar to the bone marrow of mammals. The aims of the study were to elucidate the effect of TNAP on zebrafish kidney development and function. After TNAP morpholino injection, the zebrafish embryogenesis was delay, the width and length of eyes and yolk extension were smaller and shorter than WT control, respectively. Besides, by ALP substrate staining, the expression of ALP was decreased. Using o-dianisidine staining, the hematopoiesis of RBC was decreased. By neutral red staining, the macrophages were increased in the retina and in the posterior of trunk. ALP MO was co-injected with p53 MO to exclusion the possibility that the increased number of macrophages were caused by the p53 over-expression. In conclusion that ALP plays very important roles in kidney development and function.
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