| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 98 === In the previous study, we demonstrated that Ocimum gratissimum could inhibit HSC activation. Moreover, the purified Ocimum gratissimum also inhibited the CCl4-induced liver fibrosis.The purpose of this request research program is to determine the hepaprotective effect of Chhit-Chan-Than extract powder 10% (Ocimum gratissimum). First, we determined the toxicity of Chhit-Chan-Than extract powder 10%. After 28 days of continuous tube feeding with different dose of Chhit-Chan-Than extract powder 10%, the results of biochemistry、urine biochemistry and hematology were no change. Therefore, we suggested that Chhit-Chan-Than extract powder 10% was safety in vivo. Furthermore, we investigated the hepaprotective effects of Chhit-Chan-Than extract powder 10% in vivo. The results indicated that the CCl4-induced histopathogical changes may be prevented by the Chhit-Chan-Than extract powder 10% through reducing the intercellular collogen stack, dropping blood serum ALT and AST levels; and replying the catalase activity and GSH content (the anti-oxidative enzyme activity and the anti-oxidative ingredient). Although Chhit-Chan-Than extract powder 10% has not completed to resume the formation of liver fibrosis induced by CCl4, the suppression of Chhit-Chan-Than extract powder 200 mg/Kg was nearly similar to the silymarin. Take together; we suggested that the Chhit-Chan-Than extract powder 10% was able to prevent the liver inflammation and fibrosis.
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