Investigation of MMP-2 and u-PA expressionsinhibited by Aqueous O. gratissimum extract inhuman lung carcinoma A549 cells

• Main Authors: Mei-Hui, 柯美慧
• Other Authors: 高紹軒
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/50109918760540439282

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 98 === Lung cancer is the leading cause of death in Taiwan. Among the all types of lung cancers, adenocarcinoma is the most common (~40%) and lethal type. Metastasis is a major capability of cancer cells that causes poor prognosis, recurrence and even death. Therefore, effective inhibition of metastasis should be very helpful and useful in cancer therapy. Ocimum gratissimum Linn (OG), having been widely used in herbal medicine, has been demonstrated to possess multiple anticancer bioactivity. Our previous studies have shown that aqueous extract of OG (OGE) is able to induce apoptosis of human lung adenocarcinoma A549. In the present study, we further investigate whether OGE inhibits metastasis of A549 cells and the underlying mechanism. Cell viability assay by MTT revealed that low-dose of OGE (less than 200 µg/ml) insignificantly affected viability of A549 cells. Interestingly, the low-dose OGE treatments significantly inhibited the cell motility of A549 cells by wound healing assay, as well as reduced the activity of secreted matrix metalloproteinases (MMPs) and urokinase-plasminogen activator (u-PA) by zymography assay. Additionally, we also investigated signaling pathways associated with expression of MMPs and u-PA, which showed that the low-dose OGE treatments inhibited phosphorylation of AKT but enhanced that of ERK. In conclusion, our findings indicate that low-dose OGE significantly attenuates mobility of lung carcinoma cell A549, which may attribute to inhibition of secreted MMP-2 and u-PA by low-dose OGE. It is suggested that low-dose OGE is beneficial to a combination treatment against lung cancer metastasis.