| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 98 === Cancer is often caused by disturbance in the regulation and/or execution of programmed cell death (PCD including apoptosis and autophagy) which is a complex process involving many genes and interplay between different pathways. In order to understand further the pathological roles of PCD in colorectal cancer, we used RT-qPCR array
technique to quantitatively analyze the mRNA levels of 33
apoptosis ,autophagy and angiogenesis related genes involved in pro- and anti-action of the pathways in 15 paired (tumor and non-cancerous parts)colorectal samples using GAPDH as the reference gene.In tumor tissue GAPDH mRNA content was significantly higher than that of the paired non-cancerous part with an average increased fold
of 4.01. The absolute mRNA levels for most of the 33 genes were higher in the tumor tissue also. After normalization with GAPDH Ct, the expressions of apoptosis and autophagy the related genes, except DRAM, were down-regulated in the tumor tissues statistical significantly or non-significantly. Correlation analysis revealed that the expression of most of the genes involved in the same pathway was closely related to each other in both tumor tissues and non-cancerous tissues, and that the
correlation of TNFR (in apoptosis) and Akt (in autophagy) to other genes in the same pathway was increased in tumor tissues. The correlations of the levels between angiogenesis factors and the genes of these two PCDs
were expressions of angiogenesis factors in tumor tissues are in favor of inhibiting apoptosis. Our results indicated that the level of GAPDH expression might reflect the metabolic state of cancer cells, and the regulation of PCDs in cancerous cells might have to be explained as a relative phenomenon in stead of an absolute value. The relative suppression of PCDs in tumor tissue is supposed to be in favor of cancer cell survival. Signals conducting through TNFR and Akt might contribute to the modulation of PCDs during cancerous process. Our findings provide new evidences
concerning both types of PCD that are informative to cancer research, especially in colorectal cancer.
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