| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 98 === Consumption of wasabi has many advantages such as increasing appetite, improving digestion and absorption and inhibiting bacteria and fungi in food. Previous research indicated that some components of wasabi can inhibit the proliferation of cancer cell. This study demonstrated the cytotoxic effect of wasabi (water extracts) on Coco-2 and COLO 205 cells. The results of DAPI and AVOs stains revealed that Caco-2 and COLO 205 cells both showed chromation condensation after wasabi treatment, and COLO 205 also formed AVOs, indicating that apoptosis was the major cell death mechanism caused by wasabi. Flow cytometry analysis demonstrated that low doses of wasabi (water extracts) induced growth arrest of COLO 205 cells at G2/M phase. High doses of wasabi, on the other hand, induced apoptosis of COLO 205 cells as evidenced by Annexin V and PI staining. Real time PCR analysis of the mRNA levels of apoptosis and autophagy related genes showed that both pathways were induced by wasabi extract in COLO 205 cells, and the expressions of some genes were dose-dependent to wasabi concentrations. Furthermore, a synergistic cytotoxic effect was observed when low dose of wasabi (100μg/ml) was combined with radiation. In nude mice experiment, wasabi alone did not suppress the growth of tumour. However, a combination of wasabi and radiotherapy delayed tumour growth significantly. Taken together, our studies demonstrate the anti-cancer function of wasabi in colorectal cancer and support the application wasabi as chemoprevention functional food.
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