Evaluation of antioxidant and anti-inflammatory of fifteen kinds of Chinese herbal medicine

• Main Authors: Hsiao -Ting Hu, 胡筱婷
• Other Authors: Bor-Sen Wang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/33009927065843481799

碩士 === 嘉南藥理科技大學 === 藥物科技研究所 === 98 === In recent years, people pay more attention on the health issues, therefore, the development and application of the Chinese herbals have become the world trend. In this study, 15 Chinese herbal medicine water extracts including Schizonepeta tenuifolia, Vitex trifolia, Saposhnikovia divaricata, Forsythia suspensa, Lonicera japonica, Inula britannica, Anemarrhena asphodeloides, Cimicifuga heracleifolia, Lycium chinense, Arctium lappa, Ligustrum lucidum, Perilla frutescens, Xanthium strumarium, Notopterygium incisum and Magnolia biondii were conducted to determine their antioxidant and anti-inflammatory effects. In Part 1, we focused on the studies of the antioxidant activities, include the scavenging effect on α,α-diphenyl-β-picrylhydrazyl(DPPH) radicals, the trolox equivalent antioxidant capacity (TEAC), reducing power, ferrous ion chelating power, inhibition NO capacity, scavenging superoxide radical capacity and inhibitory effect on lipid peroxidation total polyphenols and flavonoids. The HPLC-UV finger print chromatogram and antityrosinase effects of the potent herbs were also measured. In part two, we investigated the effects of Chinese herbals, on RAW 264.7 macrophages production NO induced by LPS. The results show that all these herbal extracts demonstrate antioxidant abilities and in dose-dependent manner. Forsythia suspensa, Inula britannica, and Vitex trifolia have the greatest DPPH radical scavenging effect. Inula britannica, Vitex trifolia and Cuscuta chinessis have better antioxidative activity by TEAC. The ferrous ion chelating capacity of the Forsythia suspensa, Inula Britannica and Vitex trifolia have the best effect. The reducing power of the Forsythia suspensa, Inula Britannica and Vitex trifolia have the better effect. Inhibition NO capacity of Forsythia suspensa, Inula Britannica and Vitex trifolia have the better inhibition effect. Scavenging superoxide radical capacity of the Forsythia suspensa, Inula Britannica and Vitex trifolia have the greatest superoxide radical scavenging effect. Forsythia suspensa, Inula Britannica and Vitex trifolia have the better effect of inhibitory effect on lipid peroxidation. Forsythia suspensa, Inula Britannica and Vitex trifolia had total polyphenol contents (226.5 mg、210.7 mg and 139.9mg of gallic acid equivalent, respectively) and total flavonoid contents (126.4 mg、125.2mg and 82.1mg equivalent of rutin, respectively). Forsythia suspensa, Inula britannica and Vitex trifolia exhibit potent inhibitory effects on tyrosinase. A high performance liquid chromatography analysis revealed that phenolic acids and flavonoids, such as quercitrin, rutin, chlorogenic acid, kaempferol, vanillic acid, luteolin and ferulic acid . We found that six extracts of Chinese herbs could inhibit nitric oxide generation induced by LPS by 50% or more in RAW 264.7 cells at a concentration of 400µg/ml including Inula britannica, Forsythia suspensa, Saposhnikovia divaricata, Ligustrum lucidum, Perilla frutescens and Lonicera japonica. Inula britannica has the best inhibitory effect. We found that four extracts of Chinese herbs could inhibit ROS production of RAW 264.7 cell including Lonicera japonica, Notopterygium incisum, Anemarrhena asphodeloides and Forsythia suspense. That seven extracts of Chinese herbs could inhibit ROS production generations induced by LPS in RAW 264.7 cell including Schizonepeta tenuifolia, Anemarrhena asphodeloides, Cimicifuga heracleifolia, Arctium lappa, Lonicera japonica, Xanthium strumarium and Notopterygium incisum.