The anti-proliferative effect of supercritical carbon dioxide Salvia miltorrhiza extract and its molecular mechanisms of actin in 3T3-L1 cells

• Main Authors: Shu-Jung Lee, 李淑蓉
• Other Authors: Shu-Jing Wu
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/26420658035960062997

碩士 === 嘉南藥理科技大學 === 營養與保健科技研究所 === 98 === Obesity has been a health problem in developed countries. It is a metabolic disease that usually occurs with diseases such as Type 2 diabetes mellitus, hypertension and dyslipidemia. Salvia miltiorrhiza which belongs to the Labiatae family, has been widely used in Chinese traditional medicine. It is a folk treatment for heart disease, hepatitis, haemorrhage, menstrual disorders, miscarriage, oedema, and insomnia, and recent data suggest its utility for substance dependence. In this study, our objectives were to examine the anti-proliferative properties of different Salvia miltorrhiza ( SM ) extract 〔i.e. equeous ( HWESM ), ethanolic ( EESM ) and supercritical carbon dioxide ( SCESM-5 )〕in 3T3-L1 adipocytes. Results showed that compared with HWESM and EESM, SCESM-5 demonstrated the most potent inhibitory effect on 3T3-L1 cell proliferation and cell cycle progression. After 24 h of treatment, SCESM-5 induced the generation of reactive oxygen species ( ROS ), displayed cell cycle arrest at S phase and was found to decrease in G0/G1 phase in a dose-dependent pattern. It also down-regulated the Bcl-2 and PPAR-γ proteins, and up-regulated the CD95 ( APO-1/CD95 ), Bax, AMPK and p-AMPK proteins, as well as cleaving the poly ( ADP-ribose ) polymerase ( PARP ) protein in a dose- and time-dependent manner. Furthermore, both the ERK and JNK were activated and phosphorylated after SCESM-5 treatment. Taken together, the present study indicates that SCESM-5 possesses potent anti-proliferative effects on 3T3-L1 adipocytes, and its mechanisms of action could be mediated through the CD95 ( APO-1/CD95 ) system and AMPK pathway.