Analysis of specific nucleotide fragments in bacterial infection in Kuo General Hospital

• Main Authors: Miaw-chi Peng, 彭妙琪
• Other Authors: Kun-hsiang Liu
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/65512634160693412910

碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 98 === The ability to accurately detect and identify microorganisms that are capable of causing infectious diseases has become increasingly important in environmental surveillance, clinical medicine, and biodefense settings. Bacteria infection may emerge as a serious infection for patients. Rapid identification of pathgens is required so that appropriate therapy can be given and outbreaks controlled. Standard culture and susceptibility tests permit pathogen identification and antimicrobial susceptibility profiling, but they are laborious, time-consuming, and expensive and required labile natural products. More importantly, the biochemical and serologic tests that are routingly utilized for pathogen identification only in the species or serogroup level and do not directly characterize virulence factors. In this study, 1016 bacterial clones were isolated from 917 patients. By biochemical detection, there were 38 gram negative bacteria species and 15 gram positive species. The rDNA genes are ideal genetic targets that can be used for bacterial identification, since these sequences are highly conserved between species. The intergenic spacer (ITS) region between the 16S and 23S rDNA genes is a good candidate for bacterial species identification. ITS sequence-based identification is reliable and provides a promising tool for elucidation of the clinical significance of the different species. Polymerase chain reaction (PCR)-based methods offer a distinct advantage for the detection of fastidious and noncultivable organisms. By using PCR technology, it can reduce decidable the mold identifiable spawn effectiveness (by 24 hour reduction as 4 hours), coordinate this laboratory every half year display antibiotic sensation experiment results, this method takes advantage of the results and the antibiotic feeling test results, provides clinician the preliminary news, can treat the fellow patient (complete report by 48 hour reduction is as soon as possible 24 hours). Another microorganism raise inspection produces the medical waste, according to this research experimental technique, may cause the traditional biochemical experiments which use originally to decide method produces the medical waste, by the approximately 6 kilograms reductions to the approximately 3.5 kilograms, reduced 42% infectious medical reject largely every day. These results indicate that the analysis of nucleic acid fragments is fast and simple, it provides a better method for identify the species of bacteria in order to improve the quality of therapy.