| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 98 === The classification of microalgae is complicated and mixed. The morphology of microalgae could vary with different culture conditions, these cause the classification of microalgae more difficult. Recently, many studies used molecular biology method to identify further phylogenetic relationship of algae, 18S rDNA sequencing techniques for identification basis is the most common. Therefore, this study focused on the isolation, identification and phylogenetic relationship with 18S rDNA gene sequence techniques of microalgae Scenedesmus (Chlorophyceae) taken from Kaoshiung Jiading fish farm. The result showed the native fish farm microalgae Scenedesmus: Scenedesmus AELCNU-JDB1 008; Scenedesmus AELCNU-JDB1 009 in Kaoshiung Jiading could be isolated from the by the fish farm Marine enrinchment medium f/2 fresh water medium under conditions of 32±0.5℃, and light intensity 70~80 ?慆ole photon m-2s-1. Therefore, the observation of Scenedesmus in different pH and salinity that show the morphology had changed. By the test of Nile red, these two Scenedesmus don’t have any oil reaction. In the morphology identification, they may be Scenedesmus linearis var. linearis and Scenedesmus acuminatus var. actiformis.
The analysis of molecular biotechnology of the 18S rDNA gene sequence, the sequence is about 1700bp. The identification of NCBI web site, those were Scenedesmus, but couldn’t identify the genus level by 18S rDNA gene sequence.
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