The Voltage-gated Sodium Channel Gene Mutation in Southern Taiwan Aedes aegypti (L.)

• Main Authors: Wei-lun Tseng, 曾偉倫
• Other Authors: Yi-pey Luo
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/28940900264815010360

碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 98 === Bioassay were carried out the cylinder test to evaluate the efficiency of electric mosquito liquid vaporizer of Prallethrin, Esbiothrin, Transfluthrin, Metofluthrin and d-Allethrin to NS, KM, LY and LYPR strains of Aedes aegypti in southern Taiwan. In knockdown experiments, the KT50 of NS strain were significant shorter than the other strains and the KT50 of LYPR strain were significant longer than the other strains. The mortality rates of various strains in each electric mosquito liquid vaporizer were different. Part of the S6 hydrophobic segment of domain II of the voltage-gated channel gene was obtained from NS and LY strains. Three silent polymorphisms were identified at positions, 600(A/G), 663(A/G) and 2622(A/G). These silent polymorphisms corresponded to a codon encoding lysine, leucine and glycine. The genomic DNA sequence of NS and LY strains of Aedes aegypti were compared to ensure the presence of two amino acid substutions. Our results showed that the two amino acid substation mutations of LY strain were V1023G and D1794Y. The two voltage-gated sodium channel mutations, V1023G and D1794Y, were selected for primer/probe design to carry out the TaqMan assay. The new high-throughput assay was sensitive to detect the mutations associated with insecticide resistance for resistance management strategies. Microplate assays were survey to measure levels of insensitive acetylcholinesterase, glutathione-S-transferase, α-esterase, β-esterase and monooxygenase activity to assess the insecticide resistance mechanism.