Production of polyclonal antibodies against sea bass leucocytes and cytokines induced by E.coli DNA

• Main Authors: Po-i Lin, 林博毅
• Other Authors: Kuo-Yu shen
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/79758179472361948226

碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 98 === Bacterial genimic DNA contains unmethylated CpG motifs can activate the vertebrate immune system, and it has been reported CpG oligodeoxyribonucleotides may activate the osteichthyes immune system effectively. Previous study in our laboratory showed E.coli DNA was able to induce sea bass(Lates calcarifer) head kidney cells to proliferate and produce proliferation inducing factors (PIFs). In this study, polyclonal antibodies were produced to neutralize the PIFs produced by E.coli DNA treated sea bass head kidney cells. When PIFs were depleted, the proliferation responses induced by PIFs supposed to be suppressed. The polyclonal antibodies(pAbs) obtained from immunized mice could form a clear precipitation line in double immunodiffusion. Antibodies also recognized head kidney cell surface markers and successfully neutralize the effect of PIFs. PIFs are able to be purified and sequenced by monoclonal antibodies for identification in the future.