Kinetic study on the cell of Eriobotrya japonica in suspension and bioreactor culture

碩士 === 中國醫藥大學 === 藥物化學研究所碩士班 === 98 === The aim of this study is to investigate the relation in nutrient consumption and secondary metabolites kinetics on the suspension culture of Eriobotrya cells. On the previous study, we had established the optimal culture state in loquat tissue culture. However...

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Main Authors: Kuang-Yao Liang, 梁光耀
Other Authors: Jib-Bin Wu
Format: Others
Language:zh-TW
Published: 2010
Online Access:http://ndltd.ncl.edu.tw/handle/30182656421952513035
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spelling ndltd-TW-098CMCH55490122015-10-28T04:07:29Z http://ndltd.ncl.edu.tw/handle/30182656421952513035 Kinetic study on the cell of Eriobotrya japonica in suspension and bioreactor culture 枇杷細胞震盪培養及生物反應器培養的動力學研究 Kuang-Yao Liang 梁光耀 碩士 中國醫藥大學 藥物化學研究所碩士班 98 The aim of this study is to investigate the relation in nutrient consumption and secondary metabolites kinetics on the suspension culture of Eriobotrya cells. On the previous study, we had established the optimal culture state in loquat tissue culture. However, the solid culture has low efficiency and hard to analysis. In the study, we analysis the kinetic factors between cell growth and metabolism of Eriobotrya cell. Also, the 5L bioreactor culture was established. To induce the suspension cells and high productivity cell line, and establish the analysis methods of scale inorganic elements. The cell weight was recorded, the determination of residual sugar, ions in the medium and secondary metabolites contain were carried out. In 28 days cultivation, the cell weight accumulation increased stopped on day 20, while the secondary metabolites started to accumulate rapidly on day 20. According to the result and data previously, the ions concentration, residual sugar and conductivity had a high relativity with cell dry weight and secondary metabolites production. Especially, the conductivity provides a rapid and convenient way to figure out the growth phase of cell cycle in the cultivation system. In the bioreactor experiment, the result showed that the bioreactor culture has a shorter culture period, better cell growth and better secondary metabolites accumulation. Moreover, the results of kinetic studies in shaker culture and bioreactor had high relativity. It pointed out that suspension culture of Eriobotrya cells is possible to scale up and achieve the commerciality. Jib-Bin Wu 吳金濱 2010 學位論文 ; thesis 149 zh-TW
collection NDLTD
language zh-TW
format Others
sources NDLTD
description 碩士 === 中國醫藥大學 === 藥物化學研究所碩士班 === 98 === The aim of this study is to investigate the relation in nutrient consumption and secondary metabolites kinetics on the suspension culture of Eriobotrya cells. On the previous study, we had established the optimal culture state in loquat tissue culture. However, the solid culture has low efficiency and hard to analysis. In the study, we analysis the kinetic factors between cell growth and metabolism of Eriobotrya cell. Also, the 5L bioreactor culture was established. To induce the suspension cells and high productivity cell line, and establish the analysis methods of scale inorganic elements. The cell weight was recorded, the determination of residual sugar, ions in the medium and secondary metabolites contain were carried out. In 28 days cultivation, the cell weight accumulation increased stopped on day 20, while the secondary metabolites started to accumulate rapidly on day 20. According to the result and data previously, the ions concentration, residual sugar and conductivity had a high relativity with cell dry weight and secondary metabolites production. Especially, the conductivity provides a rapid and convenient way to figure out the growth phase of cell cycle in the cultivation system. In the bioreactor experiment, the result showed that the bioreactor culture has a shorter culture period, better cell growth and better secondary metabolites accumulation. Moreover, the results of kinetic studies in shaker culture and bioreactor had high relativity. It pointed out that suspension culture of Eriobotrya cells is possible to scale up and achieve the commerciality.
author2 Jib-Bin Wu
author_facet Jib-Bin Wu
Kuang-Yao Liang
梁光耀
author Kuang-Yao Liang
梁光耀
spellingShingle Kuang-Yao Liang
梁光耀
Kinetic study on the cell of Eriobotrya japonica in suspension and bioreactor culture
author_sort Kuang-Yao Liang
title Kinetic study on the cell of Eriobotrya japonica in suspension and bioreactor culture
title_short Kinetic study on the cell of Eriobotrya japonica in suspension and bioreactor culture
title_full Kinetic study on the cell of Eriobotrya japonica in suspension and bioreactor culture
title_fullStr Kinetic study on the cell of Eriobotrya japonica in suspension and bioreactor culture
title_full_unstemmed Kinetic study on the cell of Eriobotrya japonica in suspension and bioreactor culture
title_sort kinetic study on the cell of eriobotrya japonica in suspension and bioreactor culture
publishDate 2010
url http://ndltd.ncl.edu.tw/handle/30182656421952513035
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