| Summary: | 碩士 === 中國醫藥大學 === 藥物化學研究所碩士班 === 98 === The aim of this study is to investigate the relation in nutrient consumption and secondary metabolites kinetics on the suspension culture of Eriobotrya cells. On the previous study, we had established the optimal culture state in loquat tissue culture. However, the solid culture has low efficiency and hard to analysis. In the study, we analysis the kinetic factors between cell growth and metabolism of Eriobotrya cell. Also, the 5L bioreactor culture was established. To induce the suspension cells and high productivity cell line, and establish the analysis methods of scale inorganic elements. The cell weight was recorded, the determination of residual sugar, ions in the medium and secondary metabolites contain were carried out.
In 28 days cultivation, the cell weight accumulation increased stopped on day 20, while the secondary metabolites started to accumulate rapidly on day 20. According to the result and data previously, the ions concentration, residual sugar and conductivity had a high relativity with cell dry weight and secondary metabolites production. Especially, the conductivity provides a rapid and convenient way to figure out the growth phase of cell cycle in the cultivation system. In the bioreactor experiment, the result showed that the bioreactor culture has a shorter culture period, better cell growth and better secondary metabolites accumulation. Moreover, the results of kinetic studies in shaker culture and bioreactor had high relativity. It pointed out that suspension culture of Eriobotrya cells is possible to scale up and achieve the commerciality.
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