| Summary: | 碩士 === 長榮大學 === 醫學研究所 === 98 === To investigated the inhibition of cell growth in human liver tumor cells by α-phellandrene (α-PA). α-PA is a common active principle from essential oil of dietary specie or herb. And, the human liver tumor cells (J5) cultured was as an experimental model in this study. According to the results, all of the various concentrations of α-PA were significantly decrease the cell viability of J5 cells in this study (P<0.05), with a dose-dependent manner. From FACs analysis, the cell cycle distribution were not significantly change after various concentrations α-PA treatment for 24 hours. In addition, the activities of caspase 8 and caspase 3, important regulate moleculars on induce pathway of apoptosis, were not change after α-PA treatment for 24 hours in J5 cells. From Western blot examination, the expression of Bax and Bcl-2 protein was not change after α-PA treatment for 24 hours in J5 cells. Therefore, the decline of cell viability of J5 cells by α-PA may be not through the induction of apoptosis pathway. In this study, α-PA increases the content of reactive oxygen species and reducing mitochondrial membrane potential of J5 cells after 24 hours treatment. And, α-PA was significantly increase the ATP deplation of J5 cells after 48 hour treatment (P<0.05) and their NO level was significantly increased after 24 hours treatment (P<0.05). On the other hands, 50 μM α-PA were significantly increased the protein level of PI3K III, Beclin-1 and LC3 II on J5 cells (P<0.05). In these results showed that, α-PA can inhibit cell proliferation of human liver tumor cells. The mechanisms of cell death maybe involve in necrosis or autophagy, but not apoptosis.
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