| Summary: | 碩士 === 長庚大學 === 醫學生物技術暨檢驗學系 === 98 === Abstract
Clinical virology includes a wide range of rapid diagnosis assays, such as enzyme immunoassays, immunofluorescence assays, and detection of viral nucleic acids. Yet, with all these major advances in rapid viral identification, detection of the viruses by isolation continues to be the standard by which all other methods are evaluated. Previously studies show that IPS-1 is an important component of innate immune system that is tethered to the mitochondrial membrane and helps defend against infection. We hypothesize that viruses may grow better in knockdown IPS-1 cells. In this study, shIPS-1/A549 knockdown cell line was used to cultivate potential pathogens in clinical specimens that were unable to be identified by routine working profile. Our results demonstrated that 23 specimens out of 96 specimens (the original reports are negative for viral growth) were positive for cytopathic effect in shIPS-1/A549 cell line. Therefore, the cytopathic effect rate was enhanced by using the IPS-1 knock-down cells ( from 0％ to 23.96％). Moreover, shortened culture time was obtained when a comparative study was performed in the use of positive clinical specimens. Interestingly, we also found that several blood specimens can be cultivated in IPS-1 knock-down cells. In order to identify the unknown pathogens in one of the blood specimens, gene chip was applied for detection. According to the preliminary result of gene chip analysis, we found that six pathogen candidates. Molecular assays have been performing now for further identification.
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