| Summary: | 碩士 === 長庚大學 === 生物醫學研究所 === 98 === The endocytosis is an important mechanism that controlling cell growth and proliferation. Oskar has been previeously demonstrated to play a crucial role for the formation of pole plasm and maintenance the polar granules anchoring at the posterior pole of the oocyte. We have found that Endophilin B and Oskar were colocalized at the posterior pole of the oocyte by Immunofluorescent staining. The result suggested that these two molecules may be involved in the same pathway of yolk uptake. In this study, we have used Ultra-cryosection technology and Immuno-electron microscopy to further understand D-EndoB precise localization and the ultrastructures associated with endocytosis. Shown in pattern, the IEM result is suggested that D-EndoB localized at the membrane-associated structures, and may be involved in the Oskar mediated endocytosis. After quantification of D-EndoB, I found D-EndoB actually localize at vesiculotubular structures. The yolk granules have an abnormal distribution in the D-endoB mutant oocyte. In D-endoB null mutant oocyte, the yolk volume density of central region is obviously lower than that in the wild-type, and the embryo hatched ratio was also reduced. Thus, we suggested that the biological functional of D-EndoB is involved in Oskar mediated endocytosis of yolk protein from the follicle cell.
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