Immobilization of Lipase in Magnetic Microparticles Prepared by Sol-gel Method

• Main Authors: Feng Shu Chang, 張鳳書
• Other Authors: J. P. Chen
• Format: Others
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/30450910787598424541

碩士 === 長庚大學 === 化工與材料工程研究所 === 98 === Lipase from Candida Rugosa was immobilized by entrapment in sol-gel matrix with superparamagnetic nanoparticles to catalyze the transesterification in hexane. To get the optimum value of specific activity, the experiment design was utilized. This assay includes two part experiment design, which one is to forecast the optimum value of specific activity of different immobilized conditions, another is that of different reaction condition. The parameter which is taken into consideration of immobilized condition includes the molar ratio of water and silane, amount of PTMS, addition of Enzyme, amount of additive-PVA and hydrolyzed time of silane. The result showed a quite apparent influence of amount of PTMS and hydrolyzed time of silane to specific activity when proceed enzyme immobilization. The parameter which thinked to be the factor of impacting specific activity contains the amount of additional water , concentration of Geraniol and reaction temperature. The result showed a apparent raise of specific activity after the enzyme was entrapped in sol-gel matrices. It is 8 times of immobilized enzyme to free enzyme. Moreover, the immobilized enzyme exhibits great reusability and thermal stability. To sum up, the enzyme after immobilized by sol-gel matrices represents a more stable conformation and activity then free enzyme.