Global Analysis of Modifications of the Human BK Virus Proteins by LC-MS/MS

• Main Authors: Chiung-Yao Fang, 方瓊瑤
• Other Authors: De-ching Chang
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/08581171162966599784

博士 === 國立中正大學 === 化學所 === 98 === BK virus, a human polyomavirus, may cause nephritis and urological disorders in patients who have undergone renal transplantation. Little is known about the characteristics of the BK viral proteins. In the current study, BK viral proteins were characterized by immunoblotting and LC-MS/MS. The results revealed that BK virus is composed of three structural proteins, VP1, VP2, and VP3 and four cellular histones, H2A, H2B, H3, and H4. Modifications of VP1, VP2 and VP3 were comprehensively identified by LC-MS/MS. The presence of acetylation, cysteinylation, carboxymethylation, carboxyethylation, formylation, methylation, methylthiolation, oxidation, dioxidation and phosphorylation could be identified. Modifications of viral histones are different from that of cellular histones as demonstrated by Triton-Acetic Acid-Urea (TAU) gel. This is the first report providing an analysis of the global modifications present on polyomavirus proteins. The identification of these modifications of the viral proteins should facilitate an understanding of the physiology of BKV during its life cycle.