The Conjugation of Nanoprobes Containing Gold Nanorods : Their Photoacoustic Biomedical Properties
碩士 === 國立中正大學 === 化學所 === 98 === Our research aims at the development of nanoprobes containing gold nanorod (AuNR) for both photoacoustic (PA) imaging and photothermal therapy. The first type of nanoprobe is PEG-AuNR-mAb and recently we have successfully developed its modified version with enhanced...
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| Format: | Others |
| Language: | zh-TW |
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2010
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| Online Access: | http://ndltd.ncl.edu.tw/handle/16361814091415995947 |
| Summary: | 碩士 === 國立中正大學 === 化學所 === 98 === Our research aims at the development of nanoprobes containing gold nanorod (AuNR) for both photoacoustic (PA) imaging and photothermal therapy. The first type of nanoprobe is PEG-AuNR-mAb and recently we have successfully developed its modified version with enhanced imaging contrast. The primary goals in my research are of finding suitable conjugation techniques for the attachment of monoclonal antibody onto two different core nanoparticles. Other than the successful attachment of mAb and PEG, the major concern in the conjugation is to attain the preservation in both shape and dispersity of AuNRs. Both of them assure the characteristic absorption property arising from the surface plasmon of AuNR satisfies the application need. The former project is to solve a problem related to the conjugation of linear-RGD4C peptide to AuNR. They are in opposite net charges under pH~7 buffer solutions and cause a major difficulty in the conjugation. We have successfully overcome the conjugation problem by the use of sulfonated SMCC as the adapting molecule for the RGD4C peptide. The latter project is to develop the conjugation protocol to attach both Her2mAb to the new type of core nanoparticle, gold nanorod coated with non-uniform silica shell (AuNR@nu-silica). The conjugation performed at silica instead of AuNR surface. It is much different from the conjugation procedure previously reported in literature. The nanoprobes containing AuNR@nu-silica is proven to preserve the PA enhancement of ca. 8 dB comparing to the original core nanoparticles. More importantly, the new PA nanoprobes exhibit a good specific targeting ability as it in demand.
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