Effect of electromagnetic field on the secretion of ornithine decarboxylase and human brain microvessel endothelial cells by PEI/γ-PGA/PLGA NPs

• Main Authors: Sin-wei Yu, 余信衛
• Other Authors: Yung-chih Kuo
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/45174924395370275941

碩士 === 國立中正大學 === 化學工程所 === 98 === In order to improve the low permeability of the anti-human immunodeficiency virus (HIV) drug of saquinavir (SQV) in the brain. We use poly(lactide–co–glycolide) nanoparticles (PLGA NPs) as drug carriers to permeate the blood–brain barrier (BBB)and enhance HIV therapy. The PLGA NPs were stabilized by didecyl dimethylammonium bromide (DDAB) use microemulsion-diffusion method and then surface modification of poly(?-glutamic acid) and polyethyleneimine, to increase cellular uptake. However, the average particle size of NPs decreases with increasing emulsion stirring rate while loading efficiency of ?-PGA was increases as concentration of surfactant increases. Investigating BBB permeability of the in vitro model was established by human brain microvessel endothelial cells (HBMEC) and human astrocytes (HA) co-culture on the transwell followed by study of its impact after exposure to electromagnetic field (EMF). Our results showed that the BBB permeability of SQV increased 7.4–20 folds in present of ?-PGA/PLGA NPs. In addition, enhancement of SQV permeability on BBB by AM was more significant than that by FM. This combination would be beneficial to the clinical application on the therapy of AIDS and other brain-related diseases.