| Summary: | 碩士 === 元智大學 === 化學工程與材料科學學系 === 97 === The Nile-red staining method was applied to screen potentially strains of high lipid content. A mutant Rhodococcus opacus M15009 derived from random mutagenesis of wild-type strain was found to achieve 62% w/w of lipid accumulation through two-stage cultivation. However, this mutant was indicated to accumulate low concentration of lipid with glycerol supplement. A local oleaginous microorganism was discovered capably to yield high lipid content. It was identified using 16s rRNA and 97% alliance to Staphylococcus sp. The optimization study of high cell density and lipid accumulation of Staphylococcus sp. TSP01 was carried out in respect of temperature, pH, carbon source and dissolved oxygen. A 3.43 g L-1 of biomass can be reached in the first-stage of cultivation conditions (i.e. temperature at 30℃, pH value of 8.3 and 200 rpm of stirring speed). Using glycerol as carbon source was found to yield 48.3% w/w of lipid in Staphylococcus sp. TSP01 at pH value of 5. Both of Rhodococcus opacus M15009 and Staphylococcus sp. TSP01 are concluded in characteristics as high lipid accumulation bacteria and could be applied for production of lipid feedstock in biofuel industry.
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