Characterization of polyhydroxyalkanoates- producing bacteria isolated from environment

• Main Authors: Chiao-Li Yeh, 葉巧莉
• Other Authors: 簡志青
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/40764473391308525828

碩士 === 元智大學 === 生物科技與工程研究所 === 97 === PHAs (polyhydroxyalkanoates) is biodegradability polymers which is synthesized by microorganisms, some bacteria will synthesize PHAs and accumulated in vivo when the environment have excess carbon source and lack of some nutrients (ex: nitrogen, phosphate or sulfate). PHB (polyhydroxybutyrate) is the most common type of PHAs which is accumulated by microorganisms in the environment. The enteric bacteria can be commonly found in many environments; however, there is no report on their ability for PHAs production. From complete genome sequences of some enteric bacteria (e.g. E. coli and Salmonella), there was also no evidence of genes related to PHS synthesis in their chromosomes. Most of these sequenced strains are from culture collection and clinical samples. During the process of screening PHA-producing microorganisms in the environment, we have isolated some Gram negative bacteria that can accumulate PHB and presumably belong to enteric bacteria according to their 16S rDNA sequences analysis. In this study, we examined if these environmental enteric bacteria can accumulate PHB.