Nondestructive detection of the tissue-engineered ex-vivo produced oral mucosal equivalent with Raman spectroscopy

• Main Authors: Jian-Yun Lai, 賴建允
• Other Authors: Huihua Kenny Chiang
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/9rtkx5

碩士 === 國立陽明大學 === 醫學工程研究所 === 97 === Oral surgeons have problems to deal with the huge epithelial defects after surgical intervention. Traditionally, autografts of split-thickness skin graft and/or full-thickness skin graft are used and there would be lots of complications, such as donor site morbidity, graft contractility, etc. Tissue engineering based on the patient-self oral keratinocytes with serum-free environment was developed to provide safe and sufficient tissue for defect repair and regeneration. Using Raman spectroscopy could perform the quality control of the ex-vivo tissue construct with Nondestructive and non-contact detection. This study surveyed the ex-vivo produced tissue construct with non-invasive Raman spectroscopy. For the growth of oral tissue, it avoided staining and exposured to pollution which could be able to monitor the quality of the growth of maturity. We observed that in Day1, Day7, Day14 and Day21, the peak area ratio between Amide I (1660cm-1) and Phenylalanine (1004cm-1), with the number of days increased, the ratio gradually declined. The correlation coefficient was R2 = 0.95. The lower the ratio the more mature the relationship between the tissues could be used as the basis of maturity monitoring.