Effects of Transforming Growth Factor-beta on Hyaluronan Synthase Expression in Human Breast Carcinoma Cells

• Main Authors: Shin-Chwen Han, 韓欣純
• Other Authors: Hwai-Shi Wang
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/69937013556407372355

碩士 === 國立陽明大學 === 解剖暨細胞生物學研究所 === 97 === Transforming growth factor (TGF-beta) is a secreted polypeptide and regulates cellular functions, such as cell proliferation, differentiation, migration and extracellular matrix (ECM) deposition. Also, TGF-beta can promote breast carcinoma cells migration. There is already known that overexpression of hyaluronan synthase in breast carcinoma cells will synthesize high level of hyaluronan. Because high level of hyaluronan will also promote tumorigenicity and metastasis of breast carcinoma cells, we want to understand that if there is a correlation between hyaluronan and TGF-beta. We studied in three human breast carcinoma cell lines : MCF7, MDA-MB-231, Hs578T. We used real timr-PCR, western blot, HA ELISA to study TGF-beta- induced different isoform hyaluronan synthase and hyaluronan expression in transcription level and protein level. We demonstrated that TGF-beta increase HAS1, HAS2 expression and hyaluronan synthesis in MCF7 cell line and increase HAS2 expression and hyaluronan synthesis in Hs578T cell line. These results suggest that TGF-beta promoted breast carcinoma cells migration may be related to TGF-beta induced HAS1, HAS2 expression and produces high molecular weight hyaluronan in breast carcinoma cell line.