| Summary: | 碩士 === 國立陽明大學 === 生理學研究所 === 97 === The purpose of this investigation was to establish a non-invasive, simple and efficient method to detect mouse pathogens, and to eliminate pathogens without interrupting users’ experiments.
In 2007, the mouse parvovirus (MPV) was included in the specific pathogen free (SPF) list and health monitoring services by the National Laboratory Animal Center (NLAC). The MPV infection in the animal facility was first detected in the sentinel mice by our routine health monitoring program in May, 2007. The infected mice were then identified cage by cage by ourselves by ELISA and then depopulated at the end of 2007. In 2009, the NLAC Tainan began to provide high quality laboratory mice which are MNV and Helicobacter free. However, the SPF list and health monitoring services of NLAC Taipei still did not comprise MNV and Helicobacter. Therefore, we established a fecal PCR-based method to monitor the MNV and Helicobacter in the animal facility. We found that some strains of mice in the quarantine room and holding room were infected with Helicobacter and some mice were infected with MNV in the holding room.
We used neonatal transfer in the same room for rederivation. The cages for foster dams and the infectious dams were kept on different shelves during the cleaning procedure. Within 24 hours of birth, the contaminated litters were rinsed with hypochloric acid solution (HOCl 50 ppm) several times and then transferred to the foster dams. After 3 weeks of fostering, the mice were tested for the presence of Helicobacter spp. and MNV by fecal PCR and fecal RT-PCR, respectively. Until now, neonatal transfer cleaned MNV in 7 successful transfers, and eliminated Helicobacter infection in 11 transfers, and 5 of neonatal transfer cleaned both MNV and Helicobacter at the same time.
The MPV and MNV genomic sequence are different from these being the published. The MPV is named MPV-1f and the MNV murine nororvirus Taipei/2009/TWN.
|
|---|
