| Summary: | 碩士 === 大同大學 === 生物工程學系(所) === 97 === This thesis developed the extraction methods for extracting glucosamine from
animal by products. Hyaluronic acid was extracted from the comb of roosters. The goal is
to enhance production rate as well as the purity of hyaluronic acid. The production rate of
hyaluronic acid was 0.50% for the first extraction, 0.63% for the second extraction, and
0.13% for the third extraction. HPLC was used to determine the purity of the hyaluronic
acid. Chitin and chitosan from shrimp shell was another source of glucosamine. The
production yield was 6.91% when extracted with alkaline solution. Chondroitin from pig
ears and pig trachea was obtained with acetic acid extraction process. The production
yield from pig ears is 7.15% while the production yield from pig trachea is .07%.
The glucosamines obtained from animal were used in modification experiments. We
used the Plasma treatment method to graft glucosamine onto different materials, including
pHBV membrane and sutures. The cell proliferation and adherence of pHBV
（poly(3-hydroxybutyrate-co-3-hydroxyvalerate)）membrane also can be further
improved by plasma treatment. Biodegradable sutures, Polyglactin, grafted with chitosan
were tested to determine it’s properties. The results indicate that modified sutures has
better antiseptic property than unmodified sutures.
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