The Effects of Platelet-rich Plasma on IL-1b/TNF-a-induced human arthritis

• Main Authors: Pei-Lun Lai, 賴培倫
• Other Authors: Win-Ping Deng
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/37010390385010854095

碩士 === 臺北醫學大學 === 生醫材料暨工程研究所 === 97 === Osteoarthritis (OA) is also refered to as degenerative arthritis. The pathogenesis of OA so far remains unclear. However, risk factors such as age, physical trauma and overuse of the joints have been linked to the development of OA. Clinically, injection of glucosamine and hyaluronic acid into the damaged joints have been the most common remedy. If the above management is ineffective, joint replacement surgery may be required. In this study, we will examine the pathology of OA from two respects. hPi is a human articular chondrocyte cell line established in our previous study, and their chondrocyte specific features have been well characterized. First, we used IL-1b and TNF-a to create an OA-like cell model and induced OA-related inflammatory response. At the same time, we used PRP to inhibit inflammatory response. On the other hand, we used PRP to promote the regeneration of chondrocytes. Our result showed that IL-1b and TNF-a inhibited the proliferation ability of hPi while PRP recovered it. We found the mRNA levels of Sox9, type II collagen, and aggrecan were all down-regulated when treated with IL-1b/TNF-a and up-regulated by PRP. Under our three-dimensional culture system, neo-cartilage formed in PRP-treatment group showed a higher water content and more intact gross morphology when compared to the IL-1b/TNF-a treated group. In addition, the PRP-treated group demonstrated more prominent lacunae formation and collagen matrix deposition, accompanied by a higher type II collagen expression level and GAGs accumulation. Collectively, these findings indicated that PRP could promote cartilage regeneration and potentially be an effective agent in the treatment/prevention of OA.