The Study of Intranasal Bioadhesive Liposomal-inactived-H5N3 Vaccine in Chickens

• Main Authors: Pei-Rong Jiang, 江珮蓉
• Other Authors: Der-Zen Liu
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/05311683738564397063

碩士 === 臺北醫學大學 === 生醫材料暨工程研究所 === 97 === 　　In this study, 95% PC liposomes on the activation of non-coated H5N3 influenza virus of poultry in the liposomes increased with the addition of bio-adhesive materials: Tremella, Chitosan and Xanthan gum to nasal vaccines, this vaccine can expect them to stay in the nasal cavity has reached a longer period of time the effects of continuous stimulation. 　　We used to SPF chicken animals, the first day were given first vaccination, the 14th day to give the second vaccination, after seven days sacrificed to collect the nasal washes and serum. We analyze the IgG, IgA antibody-producing capacity with ELISA to assess the vaccine to stimulate the group''s ability to generate an immune response. 　　The results were found containing bio-adhesive material of the group, which have higher serum IgG antibody. 　　Experimental use of cell performance to see the amount of nitric oxide, Xanthan gum and Liposome stimulate cells of the performance of the amount of nitric oxide, are on the rise, so Liposome and Xanthan gum that have been activated macrophages. Inhibitors see Liposome is the article to which path to go by the results showed that MEK and NF-κB pathway.