Upregulation of CXCL8 Secretion on Human Monocytes Stimulated by Connective Tissue Growth Factor

• Main Authors: Yu-Liang Lin, 林昱良
• Other Authors: 陳玫潔
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/90504932724320856488

碩士 === 臺北醫學大學 === 醫學科學研究所 === 97 === Connective tissue growth factor (CTGF), a secreted protein that modulates the fibrosis, cell adhesion, cell migration, differentiation, proliferation, angiogenesis behavior of certain cells. However, the effect of CTGF on cytokine secretion in human CD14+ monocytes is mostly unknown. Here we found that CTGF increased the secretion and expression of IL-8 (Interleukin-8) in human CD14+ monocytes. Transcriptional inhibitor (Actinomycin D) inhibited the CTGF-induced secretion of IL-8 of human CD14+ monocytes. JNK MAPK kinase inhibitors (SP600125) and p38 MAPK kinase inhibitors (SB203580) both inhibited the CTGF-induced secretion of IL-8 in human CD14+ monocytes. CTGF stimulation increased the phosphorylation of JNK and p38. In addition, we used Chromatin Immunoprecipitation to observe transcription factors AP-1 and NF-κB both binds to IL-8 gene promoter. Taken together, our results indicated that CTGF enhances the secretion of IL-8 of human CD14+ monocytes through the JNK, p38, AP-1, and NF-κB signal transduction pathway.