| Summary: | 博士 === 臺北醫學大學 === 藥學系(博士班) === 97 === The aims of this present study was conducted three parts. First part was to investigate the bioavailability in human after consuming 200 g fried purple sweet potato leaves（PSPL）. The second part was to evaluate the effects of antioxidative status, immune function and angiogenesis after consuming 200g PSPL per day for 2 weeks. The third part was to investigate the effect of crude extract from PSPL on angiogenesis of human Umbilical Vein Endothelial Cells（HUVECs）. The results showed plasma levels of polyphenols was fast increased and a peak of 1 h after ingestion of PSPL. The highest concentration of polyphenols on plasma was 1.9±0.4 μmol/L. The plasma levels of flavonols reached a peak after comsunption PSPL for 2-3 h. After 2-wk of PSPL consumption could enhance urinary polyphenol excretion by 25.2％, but did not significantly alter plasma polyphenol level. Glutathione concentration in erythrocytes were significantly enhanced by 33.3％ in PSPL group, the urinary 8-hydroxy-deocyguanosine was significantly decreased 36.7％ after 1 wk-PSPL supplementation. The consumption of PSPL produced a significant increase in proliferation responsiveness of peripheral blood mononuclear cells（PBMCs）and their secretion of immunoreactive interleukin-2 and interleukin-4, but significantly decreased the secretion of TNF-α. As well, lytic activity in NK cells and salivary IgA secretion were elevated after consuming PSPL for 2 weeks. The migration ,formation of tubular structures and the protein expression of MMP-9 were significantly increased of HUVECs treated with 5％ serum from PSPL group. The study of in vitro angiogenesis showed PSPL crude extract could inhibit HUVECs cell proliferation and decreased tubule formation in a dose response. PSLE crude extract also significantly decreased metalloproteinase-2（MMP-2）and vascular endothelial growth factor receptor（VEGFR）protein expression. In vivo study showed mild increased angiogenesis after feeding PSPL.
In conclusion, the polyphenols of PSPL was bioavailable and consuming PSPL for 2 weeks could enhance antioxidative status, immune function and angiogenesis. High concentration PSPL extract could inhibit VEGF-mediated angiogenesis.
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