Preparation of Chitosan-based tissue ngineering scaffold suitable for drug delivery application

• Main Authors: Chin-Tsung Yeh, 葉志宗
• Other Authors: Hsin-Yi Lin
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/4cvdx6

碩士 === 國立臺北科技大學 === 化學工程研究所 === 97 === It’s known that crosslinking of chitosan can made beads and films to treat as carrier of drug delivery system. Or made to porous scaffold and create a stiffer surface to enhance cell attachment and proliferation. Porous scaffold also can treat to as carrier of growing factor, anti-inflammation drug and anti-bacterial drug for in vivo experiment, to study drug delivery behavior. But less bypast research were study to drug release efficiency and drug working time. The purpose of ours research was to modify porous chitosan (C) tissue culture scaffolds and to make them suitable for controlled drug release. Chitosan (C) and chitosan differentiate to crosslinking with alginate (A), pectin (P) and genipin (G) in different proportions were made into porous scaffolds and thin films. FT-IR and thermal gravimetric analysis were used to define the type of crosslink. The hydrophility (contact angle) was tested as plate. Morphology (SEM), mechanical property (Young’s modulus) and enzymatic degradation of the CS hybrid scaffolds were measured. In the experiment, anti-inflammation drug [Pentoxifylline (PTX)] was to take as the model drug to determine their controlled release properties (swell ratio, drug release efficiency and anti-inflammatory test) and biological property (cell adherent) was tested as well. Amide (N-C=O) bonding was seen in FT-IR spectra of C/G hybrid scaffold. Amine/Carboxyl mixing peak was seen in FT-IR spectra of C/A and C/P scaffold. And the strength of signals was change with crosslinking proportion. New decomposition temperature of C/A and C/P were between C and crossslinking agents. That was confirmed the driving force of crosslinking with ALG and PEC were ionic attraction, and made ionic bond. Because the covalent bond between C and G, C/G had higher decomposition temperature. The C/A and C/P scaffolds had smaller pores and a more compact structure than the CS scaffolds. The pores of C/G scaffolds were similar to CS scaffold. C/A and C/P were more hydrophilic, but C/G was less hydrophilic. All Chitosan hybrid scaffolds were mechanically stronger (higher Young’s modulus) and less susceptible to enzymatic degradation than chitosan scaffolds. Due to the hydrophilicity, osteoblast (7F2) adhered of C/A and C/P was faster than the chitosan films. But C/G was slower than chitosan films. On the controlled release perspective, as a result of the crosslinking and the charge of 3-D structure scaffold and relation of hydrophility, Chitosan hybrid scaffolds had lower swell ratio and lower drug release efficiency than chitosan scaffolds. In anti-inflammatory test, due to the slow release property of hybrid scaffolds, the efficacy of drug were be enhanced. The anti-inflammation drug can restrain inflammation helpful In summary, the Chitosan hybrid scaffolds were better for slow down the anti-inflammation drug release efficiency. And lengthen the working time of drug release.