Measurement of Trace Amounts of Beryllium in Muscles of Poultry and Live-stock by Graphite-Furnace Atomic Absorption Spectrophotometry using Acetylacetone (acac) as a Chelating Agent

• Main Authors: Tsai Ya-Yun, 蔡亞耘
• Other Authors: Kuo Mao-Sung
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/89057772945903319395

碩士 === 東海大學 === 環境科學與工程學系 === 97 === The levels of beryllium ( Be ) in muscles of poultry and live-stock maybe very low and have not been reported in muscles of poultry and live-stock certified reference materials ( e.g. BCR CRM-384 ). The purpose of this thesis is to develop a method for the determination of Be in muscles of poultry and live-stock using microwave digestion, chelating with acetylacetone（acac）, pre-concentrating by solid-phase extraction, eluting with methanol and then measured by GFAAS. Trace amounts of Be (0.004 - 0.56 ng) in a dried pork muscle sample (20 mg) could be accurately determined by graphite-furnace atomic absorption spectrophtometry (GFAAS) after treating with microwave digestion (HNO3/H2O2) at 85C for 10 min and using acetylacetone as a chelating agent in the presence of an acetate buffer (pH 6.0). The method detection limit (MDL, 3) for Be was found to be 0.19 ng/g; the calibration graph was linear up to 28 ng/g. The Be contents measured in four muscle samples (BCR CRM - 384 pork muscle, and three samples collected in Kaohsiung, Taiwan, R. O. C.) were between 2.5 and 3.0 ng/g. Good spiked recoveries (95.7 – 103.2%) were obtained for these four samples with a relative standard deviation (RSD, n = 3) ≤ 2.1%. The proposed method could be applied to measurements of Be in muscles of poultry and live-stock.