Some novel DNA binding and cleavage agents
碩士 === 東海大學 === 化學系 === 97 === This thesis reports the determination of sequence-specific binding of several synthetic peptides incorporating the XPRK motif on a complementary pair of 5’-32P-labeled pBR322 158-mer and 135-mer DNA duplexes using DNase I footprinting. Polyacrylamide electrophoresis wa...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2009
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| Online Access: | http://ndltd.ncl.edu.tw/handle/71841741992279761963 |
| Summary: | 碩士 === 東海大學 === 化學系 === 97 === This thesis reports the determination of sequence-specific binding of several synthetic peptides incorporating the XPRK motif on a complementary pair of 5’-32P-labeled pBR322 158-mer and 135-mer DNA duplexes using DNase I footprinting. Polyacrylamide electrophoresis was employed to study the concentration-dependent and time-dependent sequence-selective cleavages on two oligonucleotide duplexes in the absence of piperidine. These studies are aimed at future gene therapy and gene modulation research.
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