The Role of AtBAG4 in Embryogenesis and Fruit Development

• Main Authors: Yi-Hsuan Lin, 林怡萱
• Other Authors: Lu-Shu Yeh
• Format: Others
• Language: en_US
• Online Access: http://ndltd.ncl.edu.tw/handle/44530193206744023420

碩士 === 慈濟大學 === 生命科學研究所 === 97 === The germination of seed is regulated by many environmental factors, such as light and water. Previous studies have found that phytochrome B (PHYB) played a major role in light mediated germination, but the signaling pathway is not well characterized. We have isolated a PHYB-interacting protein, AtBAG4 from two-hybrid screenings. Previous results of BLAST search showed that AtBAG4 shares similarity with animal BAG-1 protein. Results from Atbag4 phyB-4 double mutant indicated that loss of AtBAG4 can reverse the reduced germination rate phenotype of PHYB mutant. On the other hand, overexpression of AtBAG4 not only inhibited seed germination, but also affecting embryonic development, which resulted in difficulties of isolating homozygous AtBAG4OXphyB-9 double mutant. For phytochrome-mediated hypocotyls elongation inhibition, AtBAG4 only affect this f\process under lower fluences. In summary, we purposed that AtBAG4 might be one of the negative regulators in phytochrome B-mediated germination.