| Summary: | 碩士 === 南台科技大學 === 生物科技系 === 97 === This thesis is concerned with optimization of submerged culture conditions for mycelial growth and exopolysaccharides production by Phellinus igniarius by one-factor-at-a-time and Taguchi Methods. The one-factor-at-a-time method was adopted to investigate the effects of medium components and environmental factors on mycelial growth and exopolysaccharides production. Among these variables maltose、 yeast extract、KH2PO4、 K2HPO4、 MgSO4 were identified to be the most suitable carbon、nitrogen and mineral sources respectively. The optimal initial pH for mycelial growth and exopolysaccharides production was 5. Subsequently, the concentrations of maltose、 yeast extract、KH2PO4、 K2HPO4、 MgSO4 were optimized using the Taguchi methods. The effects of media composition on the mycelial growth of Phellinus igniarius were in the order of yeast extract＞maltose＞K2HPO4＞KH2PO4＞MgSO4 and those on exopolysaccharides production were in the order of yeast extract＞maltose＞K2HPO4＞KH2PO4＞MgSO4 . The optimal concentration for enhanced production were determined as maltose 1 %, yeast extract 0.6 %, MgSO4 5 mM, K2HPO4 10 mM, KH2PO4 2 mM for the mycelium and maltose 1 %, yeast extract 0.6%, MgSO4 10 mM, K2HPO4 2.5 mM, KH2PO4 2 mM for the exopolysaccharides, respectively. This optimal level of media compositions in shake flask culture leads to a mycelium biomass of 10.6 g/ L and exopolysaccharides production of 0.29 g/L respectively which were four times higher than that at the basal culture condition.
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