| Summary: | 碩士 === 東吳大學 === 微生物學系 === 97 === This research usage Di-(2,ethylhexyl) ester phthalate（DEHP） degrader Rodococcus zopfii strain G17. We observe the impact and influence of the bacterial community in the soil environment that polluted by DEHP. This research exploitation Denature Gradient Gel Electrophoresis（DGGE） and Community level physiological profiling （CLPP） to analysis the soil bacterial community. This research use plate count to measure the total culturable bacterium and track the Rodococcus zopfii strain G17 number with Real-time PCR counts in the soil.
We can develop after the G17 add the culturable total bacterium number increment in soil, but the increment number is not G17. And usage Real-time PCR finds that the Rodococcus zopfii strain G17 will continuously increase untile 15th day, but can't be developed on plate count.
This experiment among use the predecessors announce of the universal primers P3、 P2 carry on PCR-DGGE to observe soil bacterial communities mutually change. Match with to use Biolog GN2 microplate to monitor the
utilize ability of carbon source of bacterial communities. After analyzing through two kinds of different statistics method confirm that the result of PCR-DGGE mostly match with CLPP. These two kinds of community analysis method can really use to observe soil bacterial communities variety. Analyze the result and show to add a 100 ppm DEHP will influence bacterial communities when the testing begins but, the utilize ability of carbon source can't have immediately effect. When the 5th day the two kinds of community analysis method have no difference. When we add Rodococcus zopfii strain G17 to the microcosm, DEHP still not degradation. But that makes the bacterial communities variety.
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