The Inhibitory Effects and Application of Essential Oils on Mushroom Tyrosinase

• Main Authors: Chi-Mei Ko, 柯智美
• Other Authors: Jeng-shiow Lai
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/83nkvq

博士 === 靜宜大學 === 應用化學研究所 === 97 === In this study , the active constituent of 52 essential oils were isolated by the process of tyrosinase activity assay. The results showed that 29 essential oils showed potent inhibitory effects. It was found that the tyrosinase inhibitory activities of all the extracts increased with the increase of their concentrations. The inhibition kinetics, analyzed by Lineweaver–Burk plots, revealed that essential oil showed a mixed-type noncompetitive inhibition for mushroom tyrosinase when L-Dopa was used as substrate. A comparison of the IC50 showed that Lemongrass exhibited the most effective inhibition of tyrosinase among the essential oils. Lemongrass,Eucalyptus,Melissa,Niaouli essential oils exhibited the higher inhibitory effects and good odors。And they were formulated into the skin lotion and showed the skin whitening effects 。 Inhibitory activities of the essential oils as follows： Lemon＞Rosewood＞Orange Bitter Flower＞Chamomile Roman＞Marjoram Sweet In the complex essential oils. Lemon 80% exhibited the most significantly inhibitory activities。 Lemon,Rosewood and Orange Bitter Flower also exhibited the most significantly。 This study will help the whitening effects skin care products in the cosmetic science。It’s a good message and more safe in using whitening skin products for Asia womam。