| Summary: | 碩士 === 靜宜大學 === 食品營養研究所 === 97 === This study was to investigate the in vitro and in vivo immunostimulatory effect of konjac glucomannan (KM) and its acid-hydrolyzate fractions KMAF1 (MW >100kDa)、KMAF2 (MW10~100kDa) and KMAF3 (MW <10kDa). All KM samples prepared were composed of D-mannose and D-glucose at a ratio of 1.6:1. The in vitro results on Raw264.7 cell macrophage indicate that all KM samples examined significantly promoted the macrophage viability, where the samples of Mw > 10kDa (KM, KMAF1 and KMAF2) induced significantly production of nitric oxide, TNF-, and IL-6. By flow cytometry and ELISA, KM, KMAF1, and KMAF2 stimulated significantly the viability, surface marker expression (CD11c, CD40, CD80, CD86, and MHC II), and production of cytokines (IL-1, IL-6, IL-10, IL-12p70, TNF-), and chemokine (Rantes) of dendritic cells (DCs) from murine bone marrow. The results of mix lymphocyte reaction (MLR) for coculture of T cells and mature DCs induced by the samples illustrate that 5~100 g/mL KM and KMAF1, 10~100 g/mL KMAF2, and 50 g/mL KMAF3 significantly activated and enhanced growth of native T cells. On the other hand, KM, KMAF1 and KMAF2 at 0.3~50 g/mL and KMAF3 at 6.25 and 50 g/mL promoted significantly proliferation of murine splenocytes in vitro. In in vivo study on ICR mice, immunization of ovalbumin (OVA) adjuvant with KM, KMAF1, or KMAF2 increased significantly the IgG, IgG1, IgG2a, and IgG2b levels in serum of ICR mice, higher than those of OVA-immunized ones. In conclusion, KM samples especially of a molecular weight > 10kDa exhibited excellent abilities in activating macrophages and DCs, enhancing the proliferation of T cells and splenocytes from murine, and stimulating production of cytokines or chemokine in vitro and antibodies in vivo. KM samples are accordingly of a great potential to be used in healthy foods and as vaccine adjuvants for immunostimulatory purposes.
|
|---|
