| Summary: | 碩士 === 靜宜大學 === 食品營養研究所 === 97 === Several research studies have demonstrated herbal plants contain various potential antioxidants, and these antioxidative components may be potential antimutagens or anticarcinogens. Glechoma hederacea is belong to Labiatae family. According to the archaic Chinese prescription, Glechoma hederacea is used for medication purpose, however, little literature on its biophysical functions is presently available. This study was aimed to evaluate the antioxidative, mutagenic and antimutagenic activities of hot water extract from Glechoma hederacea (HWG). The total phenol and anthocyanin contents of extracts were also measured.
The result showed that HWG possessed antioxidative characteristics including α,α-diphenyl-β-picryl hydrazyl, 2,2‘-azino-bis (3-ethylbenzthiazoline -6-sulphonic acid) and superoxide anion radical-scavenging effects, Fe2+-chelating ability, reducing power and lipid peroxidation inhibition. It was also found that antioxidative activities of the extract increased with increasing concentractions.
The mutagenic and antimutagenic properties of the extracts were investigated using Ames test. The tester strains included Salmonella typhimurium TA97, TA98, TA100, TA102, and TA1535 with / without the metabolic activator (S9 mix). The result showed that the extract had no toxicity and mutagenicity effect toward all tester strains. The extracts (0.31~5.00 mg/plate) had marked inhibition effect against the mutagenicity of the diagnostic mutagens, 2-aminofluorene, 2-anthramine and tert-butylhydrogen peroxide, in all tester strains with the S9 mix system.
The results suggest that the hot water extracts of Glechoma headrace have antioxidative activity and it is safe in genotoxicity and exhibit the antimutagenic potential.
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