Preparation And Characterization Of Natural Antioxidant Grafted Chitosan-Silane Hybrid Scaffolds

• Main Authors: Zhen-Hong Xiao, 蕭振宏
• Other Authors: An-Chong Chao
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/03832738293693786425

碩士 === 國立聯合大學 === 化學工程學系碩士班 === 97 === Abstract Using chitosan(CS) as base material, sodium chloride particles as porogen, and (3-Chloropropyl)trimethoxysilane (CPTMS) as crosslinking agent, a novel CS/CPTMS porous hybrid (CSC1.0) was prepared. Caffeic acid was initiated by potassium persulfate to graft onto CSC1.0. SEM analysis indicated CSC1.0 and caffeic acid grafted scaffolds have porous morphology with interconnected pores and pore size ranging in 80μm to 200μm. FTIR spectra showed the covalent linkage of CS with CPTMS, and CSC1.0 with caffeic acid. DSC curve appeared water affinity of scaffolds decreased after CPTMS crosslinked, and caffeic acid grafted. The caffeic acid grafted scaffolds exhibited higher antibacterial, antioxidant property and compressive strength than CSC1.0. The degrading rate by lysozyme was in the order of CS>CSC1.0>caffeic acid grafted scaffolds. In culturing human osteosarcoma UMR-106 cells, the scaffolds showed antioxidant property-dependent inhibitions on cell growth, alkaline phosphatase activity and cell attachment of UMR-106 cells. This study suggests that caffeic acid grafting can impart compressive strength and enhance antibacterial, antioxidant and anti-cancer property on chitosan scaffold.