Fabrication And Test of an Amperometric Uric Acid Biosensor

• Main Authors: Chen-tao Chao, 趙辰濤
• Other Authors: Chia-pyng Lee
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/66359265306559854888

碩士 === 國立臺灣科技大學 === 化學工程系 === 97 === Biosensor has been considered as a kind of major method for clinic and environmental measurement due to the great sensitivity and selectivity of the utility Enzyme. An amperometric uric acid biosensor based on gold electrode was successfully developed in this research. A three-electrode strip was fabricated using thin films technique and lift-off technique to pattern the electrodes. The Ti layer and the gold layer were deposited by sputtering on the glass substrate. After that, the reference electrode and insulation layer which were used to define electrode area were deposited using thick film technique and then gold nanoparticles were deposited on the working electrode by electroplating technique. No sooner, a thioctic acid monolayer was formed by SAMs technique and the functional group of TA monolayer was activated by EDC/NHS. Eventually, Uricase was successfully immobilized on modifying the working electrode area. The range of Uric acid concentration with a good linear relation was between 0.05 to 0.5 mM. The regression coefficient of R = 0.99991, the sensitivity of the Uric acid biosensor was 37.73 μA/cm2/mM, and the average coefficient of variation was 3.68%. The interference effect from ascorbic acid (0.17mM) was observed during the interference tests. Finally, the same biosensor was observed a great stability in the first 10 days during the stability experiment.