Regulation of growth and mineralizing genes expression by sodium fluoride in pulp cells from human exfoliated deciduous teeth

• Main Authors: Yi-Wen Han, 韓依彣
• Other Authors: Min-Huey Chen
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/43012357706548844531

碩士 === 國立臺灣大學 === 臨床牙醫學研究所 === 97 === The use of fluorides in dental public health programs has a long history. Fluoride is an important and effective factor for reducing the caries incidence in children. Fluoride also increases proliferation of osteoblasts and stimulates bone formation. Exfoliated human deciduous tooth contains multipotent stem cells in the pulp tisuue. These cells were identified to be a population of highly proliferative, clonogenic cells capable of differentiating into odontoblasts. However the cellular and molecular pathways of fluoride toxicity in pulp cells derived exfoliated deciduous teeth are not very well understood. The objective of the present study was to evaluate the effects of sodium fluoride (NaF) on pulp cells derived from exfoliated deciduous teeth cultured in vitro. Pulp cells at 2.5x104cell/cm2 were incubated in vitro with NaF at 0, 10-6, 10-5, 10-4, 5x10-4 and 10-3M and MTT test was examined. Pulp cells at 2.5x104cells/cm2 in density were also incubated in vitro with NaF at 0, 10-6, 10-5, 10-4 and 5x10-4M compared to odontogenic induction medium, and then the ALP activity, calcification and mineralization were examined. A cell based quantitative evaluation of the MTT assay showed that NaF at concentration of 10-3M arrested cell growth on day 1 and day 3. Whereas the MTT assay showed that higher proliferative rate treated with NaF versus control on day 10. Alkaline phosphatase activity decreased in cells treated with NaF on day 5 and day 7 versus control. On day 14, cells treated with NaF had higher alkaline phosphatase activity than control. The gene of alkaline phosphatase expression in cells treated with NaF decreased on day 7 and day 10 versus control and induction group but enhanced on day 14. Osteocalcin expression of NaF treated cells decreased on day 7 versus control but enhanced on day 14. In conclusion, results of this study indicated that NaF modulates proliferation and mineralization pulp cells derived from exfoliated deciduous teeth proliferation and mineralization. This findings suggest that NaF may play a significant role in pulp cells physiology.