Molecular and Pathological Diagnoses on Avian Lymphoproliferative Neoplasms

• Main Authors: Kuo-Hao Chiu, 邱國皓
• Other Authors: Chen-Hsuan Liu
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/65866364732192401384

碩士 === 國立臺灣大學 === 獸醫學研究所 === 97 === Avian lymphoproliferative neoplastic diseases, especially lymphoma often occurs in a variety of avian species and have economic impact due to associated high mortality and decreased egg production rate. Most of the avian lymphomas are associated with Marek''s disease virus (MDV), avian leukosis virus (ALV) or reticuloendotheliosis virus(REV). These three viruses not only influence the poultry industry but also affect a variety of endangered species of wild birds and often lack of classical pathological diagnostic feature. Therefore, immunohistochemistry (IHC), in situ hybridization (ISH), and polymerase chain reaction (PCR) have been developed as diagnostic tools. Based on the purpose aforementioned, the aim of this study is to use PCR and IHC to differentiate the three virus-induced lymphomas. In this study, a total of 103 avian lymphoma tissue samples collected including 93 cases of paraffin-embedded tissue and 10 cases of fresh samples. All samples comprised of the nervous system, cardiovascular system, gastrointestinal system, genito-urinary system and the lymphatic system. The positive results of PCR assay show 55.43%, 21.36%, and 21.36% in ALV, MDV, and REV retrospectively. The IHC using viral antibodies shows 75.81%, 75.81%, and 61.29% in ALV, MDV, and REV retrospectively. In conclusion, IHC employed in formalin-fixed paraffin-embedded tissues of avian lymphoid neoplasms is the most effective diagnostic tool among the three diagnostic tools, especially in retrospective studies; however, viral antigens are less detected among the well-developed tumor cells of ALV or REV induced lymphomas. Establishment of ISH has successfully detected the REV nucleic acid in well-developed tumor cells in this study. By the results showed that the combination of using IHC and ISH has a great potential in retrospective study for the diagnosis of the three viruses associated avian lymphoproliferative neoplasms.