Research of functionalized nano thin-film inmicro- biosensing system

• Main Authors: Jhen-Gang Huang, 黃振剛
• Other Authors: Chii- Wann Lin
• Format: Others
• Language: en_US
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/64690867198877005267

博士 === 國立臺灣大學 === 醫學工程學研究所 === 97 === In this study, a microdevice development strategy, for which integrate the manipulation of ferrocenyl alkanethiols characteristics and idea of smart structures was investigated. The smart devices posed advantages of easy preparation and improve the device performance. However the stimuli principles of these materials might damage biomolecules and not suitable for biomedical devices development. In this study the ferrocenyl alkanethiols, for which the amphilphlic characteristics could be manipulated by electrochemical methods, was utilized for the device control. From the characterization of ferrocenyl alkanethiols, it was found doping of molecules with shorter chain length would disturbe the SAM organization, which provided more variant range. For a microfluidic device for which integrated with ferrocenyl SAM, it was proposed with interfacial hydrophilicity could be altered by oxidation of ferrocenyl SAM, which caused the fluidic infusion ratio of 50%~ 70% in converge channel. In the development of optical waveguide device, the influence of structure design in waveguide was first evaluated, after that a point-of-care kit which integrated with waveguide sensor with nitrocellulose strip for fluidic transportation was proposed. In addition, as the ferrocenyl SAM was integrated with waveguide SPR sensor, it was demonstrated that oxidation of ferrocenyl SAM would cause 2 nm shift in SPR resonance wavelength, if the SAM was doped with C4, shift magnitude of 6 nm can be achieved moreover. As the ferrocenyl SAM was integrated with SPR waveguide sensor with serial aligned multi- sensing areas. It was found the SPR signal on each sensing area might be slightly distinguished by oxidation of ferrocenyl SAM. In this study a microdevices development strategy which based on redox- active smart materials was proposed. The device performances such as fluidic manipulation efficiency and SPR signal modulation magnitude would then be improved in the future. The author wishes the research results presented can be beneficial for the development of medical and optoelectronic devices in the future.