The impacts of vinclozolin and its metabolites on soil environment

• Main Authors: Chung-Ting Lu, 盧俊廷
• Other Authors: Yei-Shung Wang
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/18687108572107203113

碩士 === 國立臺灣大學 === 農業化學研究所 === 97 === Vinclozolin has found to have the anti-androgen potential, and its metabolites, M1 and M2, are currently found to have more affinity to androgen receptors than their par-ent chemical, vinclozolin. Also, its final metabolite, 3,5-dichloroaniline, is found for more acute nephrotoxicity than other dichloroaniline stereoisomers. To monitor the de-gradation of vinclozolin and its metabolites production, and to investigate the impact of vinclozolin with its metabolites on soil microbial community, we used DNA extracted kits to collect the soil microbe’s DNA, and used solvent phase extraction to extract vin-clozolin and its metabolites. Then we utilized HPLC-UV and GC-ECD to detect vin-clozolin and metabolites in soil sample. The influences of vinclozolin and its metabolites on soil microbial community were visualized by PCR-DGGE. The half-life time of vinclozolin was 12-13 days in Pu soil and 7-9 days in Wl soil, and vinclozolin was re-sidual for more than 40 days. M1 and M2 produced in treated soils within 3 day, and they increased with time. For 48 days of incubation, M1 were amassed from 14.60 mg kg-1 to 17.77 mg kg-1 in non-sterilized soil, and from 1.35 mg kg-1 to 18.48 mg kg-1 in sterilized soil. The concentrations of Vinclozolin M2 were between 0.32 mg kg-1 and 0.91 mg kg-1 in non-sterolized soil, and between 2.16 mg kg-1 and 8.75 mg kg-1 in steri-lized soil. Additional, the degradated product M3, 3,5-dichloroaniline, could accumu-lated to 6.27 mg kg-1. Finally, the soil pH and soil microbial were the two important factors in vinclozolin degradation and metabolites production. Although vinclozolin were rapid degradated, there were other products appear. The application of vilcolzolin to soil will impact the soil microbial community, and it is important to notice the residual in our environment and to re-evaluate whether the regulation of vinclozolin has been updated.