Comparison of hexadecane utilization by bacteria isolates from coastal water of Taiwan

• Main Authors: Yin-Ting Chen, 陳盈廷
• Other Authors: 謝文陽
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/51196449493174256131

碩士 === 國立臺灣大學 === 海洋研究所 === 97 === The main composition of petroleum oil is hydrocarbon. Our laboratory isolated 29 bacterial strains which were able to utilize hexadecane as sole carbon source in 2008. After conserving strain sources for six months, six strains lost hydrocarbon-degrading ability. Four of the fastest growing bacterial strains were selected as material to study their hexadecane-utilization efficiency in three culture conditions: artificial seawater, artificial seawater with organic nutrient and filtered natural seawater. Strain HD5, HD8, HD51 and MO27 were identified as Marinobacter hydrocarbonoclasticus, Alcanivorax dieselolei, Marinobacter mobilis and Alcanivorax balearicus. Bacterial cells could adapt to new system more quickly in filtered natural seawater than in artificial seawater. Higher nitrogenous and phosphoric nutrients or organic nutrient content can improve hexadecane utilization by isolates HD5 and MO27. HD8 and MO27, classified as the same genus, had different gene sequences and number of alkB genes, and had different reactions to three culture conditions.