| Summary: | 碩士 === 國立臺灣大學 === 食品科技研究所 === 97 === Sesaminol triglucoside (STG) is the major lignan compound in sesame meal. STG can be metabolized to an antioxidant sesaminol in vivo and further into the mammalian lignans, enterodiol and enterolactone, which are reported to protect hormone-dependent diseases. Previous reports showed that intestinal microorginasms action is a key step to metabolize lignans and the intake of antibiotic can affect the conversion of lignans to enterolactone. Therefore we studied the metabolism of STG by the intestinal microorganisms of swines fed with two different kinds of feeds. Feeds containing soy peptide or antibiotic were used in this study, we hypothesize that swine fed with feed containing antibiotic as a normal animal model (antibiotic swine), and swine fed with feed containing soy peptide as a healthier animal model (peptide swine). The fresh swine feces were used as the fermentation medium for STG to monitor its metabolic transformations. We also examine the microorganisms in the feces and used the selective culture of microorganisms such as Lactobacillus, Bifidobacterium, lactic acid bacteria and Clostridium perfringens to study their biotransformation capacity of STG. Our result showed that fecal microorganisms of peptide swine can readily hydrolyze the glucose unit of STG to sesaminol and more ST-2 metabolite was formed than antibiotic swine group. We also discovered that the microorganisms, which convert sesaminol to ST-2 are sensitive to oxygen, might be the absolute anaerobes. Peptide swine feces are lower in pH value and higher in water content than antibiotic swine feces. On the other hand, antibiotic swine feces have more Lactobacillus and Bifidobacterium, but both of two groups have good ratio of good microorganisms and bad microorganisms. All of the selected microorganisms, Lactobacillus, Bifidobacterium, lactic acid bacteria and C. perfringens, can hydrolyze the glucose unit of STG, but the contribution of lactic acid bacteria is less. In conclusion, changes of diet will affect the intestinal microorganisms and therefore affect the metabolism of STG. The microorganisms present in peptide swine feces have stronger ability to metabolize STG, and we postulate that the biotransformation of STG may be due to microorganisms other than lactic acid bcteria present in the feces.
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