| Summary: | 碩士 === 國立臺灣大學 === 物理研究所 === 97 === Liver is one of the most important organs in our bodies. Meanwhile, liver disease is one of the leading causes of death in Asia, especially Taiwan. Study of liver is important and urgent. Unfortunately, liver is one of the organs lacking neuro system to report its situation. Diagnoses of liver, such as GPT, GOT, ICG, CT, MRI…etc. already solved lots of problem for human being. One of their common drawback is they don’t have real-time and in vivo microscopic analysis.
Utilizing two-photon fluorescent microscopy (TPFM) and liver chamber. We would able to monitor real-time in vivo image of movement of fluorescent probe, 6-CFDA. At the same time, we found that the metabolism of 6-CFDA could be expressed by two first-order rate equations. These equations helped us to quantify ability of influx (k1) and efflux (k2) of hepatocytes. By comparing these for parameters, we’d like to define cells’ situations or stages of whole liver. Meanwhile, zonation difference could be revealed by discussion of distribution of both parameters.
Finding the connection between macroscopic pathology and histology in previous studies and in vivo microscopic situation we monitored is our ultimate purpose.
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