| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 97 === Oxidative stress has reported would result in many diseases including chronic inflammation, skin aging and cardiovascular diseases. This study extracted powder of Gracilaria tenuistipitata by water, ethanol, methanol and methanol/HCl respectively, and obtained GTW, GTE, GTM and GTP. GTP contained the highest level of total phenolic content (221.19 ± 0.88 mg/g) and anthocyanin content (0.45 ± 0.01 μmole/g), also demonstrated the strongest DPPH radical scavenging activity and reducing power. However, GTM showed better ferrous ion chelating ability compared with other extracts. The GTW, GTE and GTM did not exert any cytotoxic effect on RAW264.7 and J774.1 cells at the highest tested concentration (1 mg/ml). But, GTP has strong cytotoxic effect. GTM remarkablely decreased superoxide anion and intracellular ROS content of macrophage induced by H2O2, LPS and AGEs, and reduced DNA fragmentation induced by AAPH. GTM increased cell viability of CCD-966SK to 166%, and collagen secretion increased by 2.4 folds. GTM, in a range from 25-200 μg/ml, was effective at protecting CCD-966SK from cell death following UV exposure, and decreased oxidative stress induced by H2O2 and UV irradiation. Taken together, these results suggest that GTM displayed antioxidant and anti-aging activities.
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